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ABSTRACT Diabetes mellitus type 2 is one of the leading causes of metabolic and cardiovascular complications. Vildagliptin is a dipeptidyl peptidase (DPP-4) inhibitor improves glycemic level. This study evaluates the antioxidant activity of Vildagliptin by its free radical scavenging activity on nitric oxide free radicals generated in the assay. Various Concentrations of Vildagliptin (100 μg/ ml, 200 μg /ml, 400 μg/ml, 800 μg/ ml, and 1000 μg/ml) was evaluated for nitric oxide free radical scavenging and the following percentage of activity was observed 45.6%, 66.97 %, 71.37 %, 84.82%, 95.49 % respectively which shows significant antioxidant activity. Key Words:- Vildagliptin, Antioxidant, NO free radical scavenging assay.

ABSTRACT Applying a dynamical system of human cognitive linguistic theory in the learning and teaching showed a relationship between learning and teaching and cognitive linguistics of theory along different dynamical systems between teachers and students in the typical university of Henan Province. The results indicated that dynamical system of cognitive linguistic theory is the most important factor affecting the learning and teaching between teachers and students. Learning and teaching were significantly correlated with a dynamical cognitive linguistic theory system in the different university along various dynamical systems between teachers and students in the typical university of Henan Province. Therefore, this dynamical human cognitive linguistic theory system plays a vital part in different dynamical systems between teachers and students of the typical university in Henan Province. Key Words: - Cognitive Linguistics of Theory, Human Ecological Theory, Dynamical System, Learning and Teaching, Typical University.

ABSTRACT One hundred and fifty (150) one –day old unsexed Ross 308 strain broiler chicks were used in this experiment to evaluate the effects of baker yeast (Saccharomyces cerevisiae) gradually replacing imported concentrate on the performance and carcass traits. Chicks were allocated into five treatment groups (A, B, C, D and E) in a complete randomized design, each group was replicated five (5) times each one with 6 chicks. Five iso-caloric, iso-nitrogenous diets were formulated, baker yeast replaced super concentrate in the ratios 0, 25, 50, 75 and 100%. Feed and water were served adlibitum. Chicks were vaccinated against gamboro. Body weight and feed consumption were measured weekly; average weight gain and FCR were calculated. Results obtained for weight gain and feed intake values were increased significantly by increasing the level of yeast replacement up to 50% then these values were decreased significantly by increasing of replacement above 50% . The mean values of feed efficiency were remained close similar between all groups. The levels of replacement had no significant effects on hot, cold dressing percentages, non-carcass yield, commercial cuts and subjective meat quality attributes and chemical composition of meat. 50% yeast replacement to concentrate obtained the highest value of marginal profit. Key Words:- Saccharomyces cerevisiae, Yeast replacement to concentrate.

ABSTRACT Although CHO cells are an excellent cellular model for studying the biophysical properties of heterologous expressed voltage-gated K+ channels, this cell line has small, but significant, endogenous K+ conductances. Here, we show that CHO cells express at least two different endogenous K+ currents: the major component was inhibited by the αKTx1 subfamily toxins Charybdotoxin (CbTX), Iberiotoxin, and Slotoxin but it was resistant to Clofilium; whereas the minor component was resistant to αKTx1 toxins. Consistently with the pharmacological profile, the current density of the main K+ current component was increased in presence of a high internal Ca2+ concentration (0.5 mM), suggesting this component is encoded by Slo1 K+ channels. The αKTx1 toxins did not affect the activation of the toxin-sensitive K+ current, but CbTX slowed the deactivation kinetics. In addition, the sequence of the αKTx1 toxins inhibitory potency suggests that native Slo1 channels are modulated, at least, by endogenous β1 ancillary subunit. The presence of native β subunits could influence the biophysical properties of heterologously expressed K+ channels in this cell line. Key Words:- Slo1 K+ channels; CHO cells; Charybdotoxin; Iberiotoxin; Slotoxin; Clofilium.

ABSTRACT

Medicinal plants in Mujib Biosphere Reserve have been evaluated. A survey for the medicinal plants over a period of two consecutive years has been conducted. A total number of 148 medicinal plant species have been recorded. The medical plants are registered based on literature previous records and knowledge gathered from contacts with local people. This number of medicinal plants form 36 % of the total number of the vascular plants enumerated in the study area (148/408), reflecting the richness of this dry ecosystem that ranges in altitude from 900 m above sea level to (-400) m below sea level at the Dead Sea borders. The largest numbers of medicinal plants are recorded in the study area are belonging to the families: Asteraceae, Fabaceae, Poaceae, Lamiaceae, Boraginaceae and Chenopodiaceae.

Key Words:- Jordan, Mujib Biosphere Reserve, Medicinal Plants.

ABSTRACT Ciprofloxacin hydrochloride is a broad spectrum anti-microbial drug, listed in class 4 of biopharmaceutics classification of drugs. Tinidazole is a prodrug and antiprotozoal agent, listed in class 2 of biopharmaceutics classification of drugs. In the present study a new dissolution medium was developed, as there is no official dissolution medium available in the literature for combined dosage form containing ciprofloxacin and tinidazole. The composition of the dissolution medium was selected on the basis of solubility data .Solubility data revealed that 0.01 N HCl may be suitable as dissolution medium. The selected dissolution medium was used for the evaluation of combined dosage form containing ciprofloxacin and tinidazole. A reverse phase high performance liquid chromatography method was developed for estimation of ciprofloxacin and tinidazole in combined dosage form. The separation was achieved by Inertsil C18 (250Ð¥4.6mm) column, 5 μm and Buffer: Acetonitrile (82:18) as eluent, at a flow rate of 1.0 ml /min. Detection was carried out at 316 nm. The retention time of ciprofloxacin & tinidazole was found to be 5.6 and 9.7 minutes respectively. Linearity was in the range of 27.5 - 82.5μg/ml for ciprofloxacin, and 33μg/ml - 66μg/ml for tinidazole. The developed method was found to be accurate, precise and selective for the development of ciprofloxacin and tinidazole in combined tablet dosage form. Key Words:- Ciprofloxacin Hydrochloride, Anti- microbial, Tinidazole, Anti-protozoal.

ABSTRACT

Dermatophytes are a group of fungi that cause infections in keratinized human and animal tissues. Physical and chemical agents can be effective in reveals of dermatophytosis pathogenesis in human which some people are sensitive and some other are resistance to it. Amino acid changes may be a risk factor for infection with dermatophytes in mammals. In the framework of a survey on the comparative changes of free amino acids in stratum corneum in 60 patient with dermatophytosis in two site, one , near skin lesion and two, sole area and 60 healthy volunteers(normal subjects), at sole area were done Amino acid in stratum corneum analyzed by HPLC method and the identification of dermatophytosis was based on direct examination and culture. The results of research statistically were analyzed by software and comparison of mean by using the t-test. Achieved results between case and control in sole area showed that cases were significantly increased in amino acids: Aspartate - Tyrosine –Tryptophane - Phenylalanine and were significantly decreased in amino acids: Citrulline– Ornithine Similarly, in two sex male and female. In also people with dermatophytosis in two site near skin lesion and sole area distribution in associated were significantly increased in Glutamates - Asparagine - Histidine - Glutamine - Arginine - Citrulline - Threonine - Methionine - Leucine – Ornithine and were significantly decreased only in Glycine. Our research shows that due to the concentration, amino acids can effect stimulation or inhibition of dermatophytes growth in stratum corneum.

Key Words:- Dermatophyte - Stratum corneum, Free amino acids .

ABSTRACT

Chronic obstructive pulmonary disease (COPD) is the most common lung disorders in adults with progressiveobstruction, defuse irreversible airway narrowing is associated with increased resistance to air. Many inflammatory andimmunological activates have role in induction of disease. Among of them haptoglobulin (Hp) as an acute phase protein mayhas key role in induction of disease. As there is no available report in connection with the study of serum haptoglobin inCOPD patients so we decided to investigate the role of serum Hp in COPD patients. This case-control study was done on 60patients with COPD and 60 healthy controls in the clinic of lung at Ali Ibn Abi Talib (AS) hospital of Zahedan. 5mls ofperipheral blood were collected from patients and healthy control and serum was prepared and haptoglobulin concentrationwas measured by nephlometry method. Among individuals who were diagnosed with COPD, the haptoglobin serum levelswas calculated as 1.89 ± 0.80 grams per liter and in people without COPD mean haptoglobin concentration was 1.19 ± 0.76grams per liter. This difference was statistically significant (p<0.0001). Thus it was concluded that ,high concentration ofhaptoglobulin has relation with COPD disease.

Key words:- Haptoglobin - COPD.