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The objective of this research is to formulate and evaluate Solid Lipid Nanoparticles containing Acyclovir by various phospholipids and surfactants using hot homogenization technique. Totally nine batches of SLN was formulated by hot homogenization technique and evaluates for PS,ZP,PI,Drug content, Entrapment efficiency, Invitro drug release studies and Invitro release kinetics studies. From the data’s, it was concluded that hot homogenization technique method followed by ultrasonication was an optimized technique for the preparation of SLN nanoparticles containing Acyclovir, which lead to better results like high entrapment efficiency, high drug content and SLS was a better choice of surfactant to reduce the particle size and leads to uniform distribution of SLN in its phase. So, SLN will be an alternative drug delivery system for acyclovir to enhance the bioavailability and therapeutic response of drug.Key Words:-Acyclovir, Solid Lipid Nanoparticles, Invitro evaluation, Particle Size (PS), Polydispersity index (PDI), Zeta potential (ZP) etc.

The main aim of the study is to develop sustained release microspheres loaded with Fenofibrate. The microsphere were developed using coacervation and phase separation method using gelatin as a polymer. Totally two formulations were developed and evaluated for FTIR, DSC, Scanning electron microscopy, percentage yield, percentage drug entrapment, invitro drug release studies. The results of the study indicate that the microspheres having good flow property (24°11’’ and 25°22’’).The particle size were ranged from 5-10μm. The encapsulation efficiency was found to be 70% for F1 formulation and 97% for F2 formulation. The invitro release study were carried out using pH 6.8 phosphate buffer for the period of 12hrs. The F2 formulation released the drug over the period of 12hrs. In conclusion, F2 formulation suitable for oral sustained release of Fenofibrate.

A simple, precise, specific and accurate reverse phase high performance liquid chromatography (RP-HPLC) method wasdeveloped and validated for determination of ezetimibe and glimepiride in pharmaceutical tablet dosage form. The differentanalytical performance parameters such as linearity, accuracy, precision, range, LOD and LOQ were determined according toICH guideliness. RP-HPLC was conducted on Hypersil BDS C18 (250 mm length x 4.6 mm ID, 5μm) column. The mobilephase was consisting of buffer (0.01M potassium di hydrogen phosphate at pH 4.8) and acetonitrile in the ratio (30:70% v/v)and the flow rate was 1ml/min. Ezetimibe and Glimepiride were monitored using WATERS HPLC 2695 SYSTEM with autoinjector and PDA detector. Linearity was observed in concentration ranges of 2.5-15 μg/ ml and 25-125 μg/ ml for Glimepirideand Ezetimibe respectively. Regression equation of Ezetimibe is y = 19217x + 1355, and of Glimepiride is y = 11306x + 2315.Correlation coefficient was found to be 0.999, 0.999 for Ezetimibe and Glimepiride respectively. The %RSD of repeatabilitywas found to be 0.3730 and 0.3577 for Ezetimibe and Glimepiride, respectively. The %RSD of inter day precision was found tobe 0.3660and 0.3501for Ezetimibe and Glimepiride, respectively. The %recovery was found to be 99.78% for Ezetimibe,99.98% for Glimepiride. LOD value of Ezetimibe and Glimepiride was found to be 0.2, 0.7, respectively. LOQ value ofEzetimibe and Glimepiride was found to be 0.7, 2 respectively. All the system suitability parameters were found within range.Key Words:-Ezetimibe, Glimepiride, RP-HPLC, Linearity,

Dicloxacillin Sodium is a Beta-lactum antibiotic. The aim of the present study is to develop a new analytical method for the estimation of Dicloxacillin Sodium in bulk and in tablet dosage form. Spectroscopic method has been developed for the quantification of Dicloxallin Sodium in bulk and in the formulation. This method is simple, cost effective, accurate and precise. In this method, Dicloxacillin Sodium showed maximum absorbance at 274 nm in double distilled water, which is selected as solvent for our analysis based on its stability. Beer’s law obeyed in the concentration range of 5-25 mcg/ ml. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.516682 and 1.565703 mcg/ml respectively. This method is validated as per ICH guidelines. The method was found to be simple, accurate, Precise and rapid.Key Words:- UV-spectroscopic method, Dicloxacillin Sodium, precise.

Autism is one of the most persistent developmental disorder. This means that most people on the autism spectrum have delays, differences or disorders in many areas including gross and fine motor skills. Children on the spectrum have low muscle tone, or have a hard time with coordination with exercise and sports. These issues can interfere with basic day by day functioning and almost hinder with social and physical development. In this review it has been discussed that Physical therapy may be an option for children with autism who need help developing age-appropriate motor skills, have low muscle tone, or have problems with physical systems such as breathing control. And also older autistic children can also benefit from carefully constructed exercise programs, which may be led by a physical therapist.Key Words:- Autism, Physical therapy, Motor skills etc.

The aim of present study was to estimate the Antioxidant activity of methanol extract of the inner bark of Guettarda speciosa L. (Rubiaceae) was assessed by using 2,2- diphenyl-1-picryl-hydrazyl (DPPH•) assay and reducing power activity. Here, ascorbic acid was used as standard antioxidant. The results of the study indicate that the methanolic extracts of the inner bark of Guettarda speciosa L. (Rubiaceae) possess significant scavenging activity against DPPH• and reducing power activity. The free radical scavenging and antioxidant activities may be attributed to the presence of adequate flavonoid compounds in methanol extract of the inner bark of Guettarda speciosa L. (Rubiaceae). This study revealed that the methanolic extract of methanol extract of the inner bark of Guettarda speciosa L. (Rubiaceae) has demonstrated significant antioxidant activity.Key Words:- DPPH assay, Reducing power activity, Guettarda speciosa L.