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Objective: The day by day new combinations drugs are being introduced in market. Then the multiple therapeutic agents
which acts at different sites are used in the management of various diseases and disorders are done. Thus it is necessary to
develop methods for analysis with the help of number of analytical techniques which are available for the estimation of
the drugs in combinations. An accurate, precise and reproducible RP-HPLC method was developed for the simultaneous
quantitative determination of Metformin Hydrochloride (MET) and Pioglitazone (PIO) in tablet dosage forms. Methods:
Younglin (S. K.) gradient system UV detector and C18 column with 250 mm x 4.6 mm i. d. and 5?m particle size
Acetonitrile: OPA water (80: 20v/v) pH 2.5 was used as the mobile phase for the method. The detection wavelength was
283 nm and flow rate was 0.9 ml/min. Results: In the developed method, the retention time of MET and PIO were found to
be 6.366 min and 8.616 min. The developed method was validated according to the ICH guidelines. Conclusion: In this
methods linearity, precision, range, robustness were observed. The method was found to be simple, accurate, precise,
economic and reproducible. So the proposed methods can be used for the routine quality control analysis of MET and PIO
in bulk drug as well as in formulations
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